pBabe EGFR Del3(T790M)
(Plasmid
#32071)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 32071 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBABEpuro
- Backbone size w/o insert (bp) 5169
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEGFR
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SpeciesH. sapiens (human)
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Insert Size (bp)3600
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MutationDelete L747-A750, insert P, T790M
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Entrez GeneEGFR (a.k.a. ERBB, ERBB1, ERRP, HER1, NISBD2, NNCIS, PIG61, mENA)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SnaB1 (destroyed during cloning)
- 3′ cloning site SnaB1 (destroyed during cloning)
- 5′ sequencing primer pBABE 5' (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBabe EGFR Del3(T790M) was a gift from Matthew Meyerson (Addgene plasmid # 32071 ; http://n2t.net/addgene:32071 ; RRID:Addgene_32071) -
For your References section:
Allele-dependent variation in the relative cellular potency of distinct EGFR inhibitors. Yuza Y, Glatt KA, Jiang J, Greulich H, Minami Y, Woo MS, Shimamura T, Shapiro G, Lee JC, Ji H, Feng W, Chen TH, Yanagisawa H, Wong KK, Meyerson M. Cancer Biol Ther. 2007 May . 6(5):661-7. 10.4161/cbt.6.5.4003 PubMed 17495523