pDAI-SceI
(Plasmid
#32061)
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PurposeConstruction of unmarked gene deletions. Broad host range replicative plasmid expressing the I-SceI homing endonuclease
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 32061 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDA17 (Broad host range plasmid recovered form Bordetella bronchiseptica)
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Backbone manufacturerDaniel Aubert
- Backbone size w/o insert (bp) 7360
- Total vector size (bp) 8031
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Tetracycline, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameI-SceI nuclease
- Promoter Pdhfr
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer pBRrevBam
- 3′ sequencing primer pBAD Reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDAI-SceI was a gift from Miguel Valvano (Addgene plasmid # 32061 ; http://n2t.net/addgene:32061 ; RRID:Addgene_32061) -
For your References section:
A system for the construction of targeted unmarked gene deletions in the genus Burkholderia. Flannagan RS, Linn T, Valvano MA. Environ Microbiol. 2008 Jun;10(6):1652-60. Epub 2008 Mar 13. 10.1111/j.1462-2920.2008.01576.x PubMed 18341581
Map uploaded by the depositor.
