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MCV-hVam6p-
(Plasmid #32059)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32059 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJ241
  • Backbone manufacturer
    DNA2.0
  • Backbone size w/o insert (bp) 2755

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MCV-hVam6p-
  • Species
    Merkel cell polyomavirus
  • Insert Size (bp)
    5387
  • Mutation
    TG to GC mutations at 3280-3281 (nt) of full construct
  • GenBank ID
    JF813003
  • Promoter none from vector. Insert contains native promoter from viral genome encompassed in the viral non-coding control region.

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsrFI (not destroyed)
  • 3′ cloning site BsrFI (not destroyed)
  • 5′ sequencing primer NA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MCV-hVam6p- was a gift from Patrick Moore (Addgene plasmid # 32059 ; http://n2t.net/addgene:32059 ; RRID:Addgene_32059)

  • For your References section:

    Cellular and viral factors regulating merkel cell polyomavirus replication. Feng H, Kwun HJ, Liu X, Gjoerup O, Stolz DB, Chang Y, Moore PS. PLoS One. 2011;6(7):e22468. Epub 2011 Jul 22. 10.1371/journal.pone.0022468 PubMed 21799863
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