PIWIL2
(Plasmid #32042)

• Purpose: Bacterial expression for structure determination; may not be full ORF
• Depositing Lab: Cheryl Arrowsmith
• Publication: SGC Q1 & Q2 2011 (unpublished)

Backbone

• Vector backbone: pET28-MHL
• Backbone manufacturer: Structural Genomics Consortium (Addgene plasmid 26096)
• Backbone size w/o insert (bp): 7325
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: PIWIL2
• Alt name: PDB: 3QIR
• Alt name: piwi-like 2, PAZ domain
• Species: H. sapiens (human)
• Insert Size (bp): 444
• Mutation: PAZ domain only (amino acids 386-533)
• GenBank ID: BC111751
• Entrez Gene: PIWIL2 (a.k.a. CT80, HILI, PIWIL1L, mili)
• Tag / Fusion Protein:
  • His (with TEV cleavage) (N terminal on backbone)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

PDB: 3QIR. http://www.thesgc.org/structures/3QIR/