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Addgene

pYES2.1-ScDcr1
(Plasmid #32036)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32036 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYES2.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5800
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Drc1
  • Species
    S. castellii
  • Promoter GAL1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer Gal1
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYES2.1-ScDcr1 was a gift from David Bartel (Addgene plasmid # 32036 ; http://n2t.net/addgene:32036 ; RRID:Addgene_32036)

  • For your References section:

    The inside-out mechanism of dicers from budding yeasts. Weinberg DE, Nakanishi K, Patel DJ, Bartel DP. Cell. 2011 Jul 22;146(2):262-76. 10.1016/j.cell.2011.06.021 PubMed 21784247
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