pRSF-KpDcr1deltaNdeltaC
(Plasmid
#32030)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 32030 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonesumo-pRSFduet-1
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 4100
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Modifications to backboneamino-terminal Ulp1-cleavable His6-SUMO
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameDCR1(107-355)
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SpeciesK. polysporus
- Promoter T7
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Tag
/ Fusion Protein
- His6-SUMO (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The species of DCR1 is Kluyveromyces polysporus, which is also known as Vanderwaltozyma polyspora
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRSF-KpDcr1deltaNdeltaC was a gift from David Bartel (Addgene plasmid # 32030 ; http://n2t.net/addgene:32030 ; RRID:Addgene_32030) -
For your References section:
The inside-out mechanism of dicers from budding yeasts. Weinberg DE, Nakanishi K, Patel DJ, Bartel DP. Cell. 2011 Jul 22;146(2):262-76. 10.1016/j.cell.2011.06.021 PubMed 21784247