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pRSF-KpDcr1deltaC(E224Q)
(Plasmid #32027)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32027 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    sumo-pRSFduet-1
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 4100
  • Modifications to backbone
    amino-terminal Ulp1-cleavable His6-SUMO
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    DCR1(15-355)
  • Species
    K. polysporus
  • Mutation
    E224Q
  • Promoter T7
  • Tag / Fusion Protein
    • His6-SUMO (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The species of DCR1 is Kluyveromyces polysporus, which is also known as Vanderwaltozyma polyspora

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSF-KpDcr1deltaC(E224Q) was a gift from David Bartel (Addgene plasmid # 32027 ; http://n2t.net/addgene:32027 ; RRID:Addgene_32027)

  • For your References section:

    The inside-out mechanism of dicers from budding yeasts. Weinberg DE, Nakanishi K, Patel DJ, Bartel DP. Cell. 2011 Jul 22;146(2):262-76. 10.1016/j.cell.2011.06.021 PubMed 21784247
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