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Addgene

pLKO-puro human lipin 1-1 shRNA
(Plasmid #32019)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32019 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLKO.1-TRC
  • Backbone manufacturer
    Addgene Plasmid 10878
  • Backbone size w/o insert (bp) 7100
  • Vector type
    Mammalian Expression, Lentiviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    lipin1 shRNA
  • Alt name
    fld; Lpin1; Kiaa0188; mKIAA0188; 4631420P06
  • gRNA/shRNA sequence
    one of the following 5: CCAGTGTTTGACAGACATCAA, GCTCTTTAATTGCTTTGGTTA, GCCCAGTTGGACAATCTCAAA, GCCGTGTCATATCAGCAATTT, CGCCAAAGAATAACCTGGAAA
  • Species
    H. sapiens (human)
  • GenBank ID
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer pLKO.1 5'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Check sequence read for actual shRNA target sequence

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLKO-puro human lipin 1-1 shRNA was a gift from David Sabatini (Addgene plasmid # 32019 ; http://n2t.net/addgene:32019 ; RRID:Addgene_32019)

  • For your References section:

    mTOR Complex 1 Regulates Lipin 1 Localization to Control the SREBP Pathway. Peterson TR, Sengupta SS, Harris TE, Carmack AE, Kang SA, Balderas E, Guertin DA, Madden KL, Carpenter AE, Finck BN, Sabatini DM. Cell. 2011 Aug 5;146(3):408-20. 10.1016/j.cell.2011.06.034 PubMed 21816276
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