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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 32018 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLKO.1-puro
- Backbone size w/o insert (bp) 7100
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Modifications to backboneU6 replaced by CMV and FLAG added N-term to insert. 5->3': CMV-> FLAG -> sal I restriction site -> 1 base(to keep in frame) -> cDNA -> Not I restriction site
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSREBP2
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SpeciesM. musculus (mouse)
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Entrez GeneSrebf2 (a.k.a. SREBP-2, SREBP2, SREBP2gc, bHLHd2, lop13, nuc)
- Promoter CMV
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Tag
/ Fusion Protein
- FLAG (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CMV forward (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLKO-puro FLAG SREBP2 was a gift from David Sabatini (Addgene plasmid # 32018 ; http://n2t.net/addgene:32018 ; RRID:Addgene_32018) -
For your References section:
mTOR Complex 1 Regulates Lipin 1 Localization to Control the SREBP Pathway. Peterson TR, Sengupta SS, Harris TE, Carmack AE, Kang SA, Balderas E, Guertin DA, Madden KL, Carpenter AE, Finck BN, Sabatini DM. Cell. 2011 Aug 5;146(3):408-20. 10.1016/j.cell.2011.06.034 PubMed 21816276