pLKO-puro FLAG SREBP1
(Plasmid #32017)

• Depositing Lab: David Sabatini
• Publication: Peterson et al Cell. 2011 Aug 5;146(3):408-20.

Backbone

• Vector backbone: pLKO.1-puro
• Backbone size w/o insert (bp): 7100
• Modifications to backbone: U6 replaced by CMV and FLAG added N-term to insert. 5->3': CMV-> FLAG -> sal I restriction site -> 1 base(to keep in frame) -> cDNA -> Not I restriction site
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SREBP1
• Species: M. musculus (mouse)
• Mutation: V225M
• Entrez Gene: Srebf1 (a.k.a. ADD1, SREBP1, bHLHd1)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV forward

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 14 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry