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Addgene

pLKO-puro FLAG wildtype, catalytic active lipin 1
(Plasmid #32010)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 32010 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLKO.1-puro
  • Backbone size w/o insert (bp) 7100
  • Modifications to backbone
    U6 replaced by CMV and FLAG added N-term to insert. 5->3': CMV-> FLAG -> sal I restriction site -> 1 base(to keep in frame) -> cDNA -> Not I restriction site
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    lipin1
  • Alt name
    fld; Lpin1; Kiaa0188; mKIAA0188; 4631420P06
  • Species
    M. musculus (mouse)
  • GenBank ID
    NM_001130412
  • Entrez Gene
    Lpin1 (a.k.a. Lipin1, fld)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV forward
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLKO-puro FLAG wildtype, catalytic active lipin 1 was a gift from David Sabatini (Addgene plasmid # 32010 ; http://n2t.net/addgene:32010 ; RRID:Addgene_32010)

  • For your References section:

    mTOR Complex 1 Regulates Lipin 1 Localization to Control the SREBP Pathway. Peterson TR, Sengupta SS, Harris TE, Carmack AE, Kang SA, Balderas E, Guertin DA, Madden KL, Carpenter AE, Finck BN, Sabatini DM. Cell. 2011 Aug 5;146(3):408-20. 10.1016/j.cell.2011.06.034 PubMed 21816276
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