pRK5 FLAG 17xS/T->A, catalytic active lipin 1
(Plasmid #32007)

• Depositing Lab: David Sabatini
• Publication: Peterson et al Cell. 2011 Aug 5;146(3):408-20.

Backbone

• Vector backbone: pRK5
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: lipin1
• Alt name: fld; Lpin1; Kiaa0188; mKIAA0188; 4631420P06
• Species: M. musculus (mouse)
• Mutation: S106A, S150A, (S281A, T282A), S285A, S287A, S293A, T298A, S328A, (S353A, S356A), S392A, S468A, S472A, S483A, S634A, S635A, (S647A, S648A)
• GenBank ID: NM_001130412
• Entrez Gene: Lpin1 (a.k.a. Lipin1, fld)
• Promoter: CMV, SP6
• Tag / Fusion Protein:
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: SP6

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The 17xS/T→ A mutant encompasses the following residues: S106, S150, (S281, T282), S285, S287, S293, T298, S328, (S353, S356), S392, S468, S472, S483, S634, S635, (S647, S648). Residues in parentheses were both mutated to alanine because the singly phosphorylated site in each case could not be unambiguously determined by mass spectrometry

S921A, S923A were previously reported to have been present in this plasmid, but these residues are in fact WT. This change should no affect the reported function.