CaM-RhoA(DP)-Venus
(Plasmid
#31994)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 31994 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepTriEx-3
- Backbone size w/o insert (bp) 5000
-
Vector typeMammalian Expression, Bacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameCaM-RhoA(DP)
-
Alt nameCalmodulin-RhoA(Q63L)
-
SpeciesH. sapiens (human)
-
Insert Size (bp)1700
-
MutationQ63L mutation in RhoA, E7S and D65Y in CaM
-
Entrez GeneRHOA (a.k.a. ARH12, ARHA, EDFAOB, RHO12, RHOH12)
-
Tag
/ Fusion Protein
- YFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer tx53 (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
CaM-RhoA(DP)-Venus was a gift from Kevin Truong (Addgene plasmid # 31994 ; http://n2t.net/addgene:31994 ; RRID:Addgene_31994) -
For your References section:
Structure based design of a Ca2+-sensitive RhoA protein that controls cell morphology. Mills E, Pham E, Truong K. Cell Calcium. 2010 Oct;48(4):195-201. 10.1016/j.ceca.2010.08.009 PubMed 20888042