pcDNA3.1(+)Flag-His-ATM wt
(Plasmid #31985)

• Purpose: Mammalian expression of human ATM with Flag and His tags
• Depositing Lab: Michael Kastan
• Publication: Canman et al Science. 1998 Sep 11;281(5383):1677-9.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5428
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl2
• Growth instructions: After transformation, grow for 2 days at 30 degrees. Ignore colonies that grow after 1 day. Let grow for a full 2 days.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ATM wild-type
• Alt name: ATI
• Alt name: ATA
• Alt name: ATDC
• Species: H. sapiens (human)
• Insert Size (bp): 9581
• Mutation: 3' UTR of 400 nucl.
• GenBank ID: NP_000042.3/71902540
• Entrez Gene: ATM (a.k.a. AT1, ATA, ATC, ATD, ATDC, ATE, TEL1, TELO1)
• Tags / Fusion Proteins:
  • Flag (N terminal on backbone)
  • HIS (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI-SalI Ligation (destroyed during cloning)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Supplemental Documents:
  • Transformation of the ATM Plasmid
• Addgene Notes:
  • Addgene Diagnostic Digest
• A portion of this plasmid was derived from a plasmid made by: ATM cDNA generated by Dr's Yael Ziv and Yossi Shiloh and with this cDNA Dr. Dae Sik Lim/laboratory of Michael Kastan sub-cloned it
• Articles Citing this Plasmid:
  • 34 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry