GFP- RPL26
(Plasmid #31980)

• Depositing Lab: Michael Kastan
• Publication: Takagi et al Cell. 2005 Oct 7. 123(1):49-63.

Backbone

• Vector backbone: pEGFP C3
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RPL26
• Alt name: L26
• Species: H. sapiens (human)
• Insert Size (bp): 1185
• GenBank ID: NM_000987.5 NP_000978.1
• Entrez Gene: RPL26 (a.k.a. DBA11, L26)
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xho1 (not destroyed)
• 3′ cloning site: Xho1 (not destroyed)
• 5′ sequencing primer: EGFP-C-

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Masatoshi Takagi Michael Kastan Laboratory St. Jude Children's Research Hospital Memphis, TN
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry