myc-hAtg13
(Plasmid #31965)

• Depositing Lab: Do-Hyung Kim
• Publication: Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003. Epub 2009 Feb 18.

Backbone

• Vector backbone: pRK5
• Backbone size w/o insert (bp): 4754
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Atg13
• Species: H. sapiens (human)
• Entrez Gene: ATG13 (a.k.a. KIAA0652, PARATARG8)
• Promoter: CMV, SP6
• Tag / Fusion Protein:
  • myc (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: SV40pA-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: ATG13 cDNA were obtained from the Katzusa Institute.
• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry