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Addgene

HA-hULK1
(Plasmid #31963)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31963 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRK5
  • Backbone size w/o insert (bp) 4754
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ULK1
  • Species
    H. sapiens (human)
  • Entrez Gene
    ULK1 (a.k.a. ATG1, ATG1A, UNC51, Unc51.1, hATG1)
  • Promoter CMV, SP6
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA-hULK1 was a gift from Do-Hyung Kim (Addgene plasmid # 31963 ; http://n2t.net/addgene:31963 ; RRID:Addgene_31963)

  • For your References section:

    ULK-Atg13-FIP200 complexes mediate mTOR signaling to the autophagy machinery. Jung CH, Jun CB, Ro SH, Kim YM, Otto NM, Cao J, Kundu M, Kim DH. Mol Biol Cell. 2009 Apr;20(7):1992-2003. Epub 2009 Feb 18. 10.1091/mbc.e08-12-1249 PubMed 19225151
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