pBabe hygro 3xFLAG Dna2 D294A (nuc)
(Plasmid #31956)

• Depositing Lab: Sheila Stewart
• Publication: Duxin et al J Biol Chem. 2012 Jun 22;287(26):21980-91. Epub 2012 May 7.

Backbone

• Vector backbone: pBabe hygro 3xFLAG
• Backbone size w/o insert (bp): 5200
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hDna2
• Alt name: Dna2L
• Species: H. sapiens (human)
• Insert Size (bp): 3200
• Mutation: Changed start ATG to AAA, and D294A
• GenBank ID: NM_001080449
• Entrez Gene: DNA2 (a.k.a. DNA2L, hDNA2)
• Tag / Fusion Protein:
  • 3xFLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PAC1 (not destroyed)
• 3′ cloning site: SAL1 (not destroyed)
• 5′ sequencing primer: pBabe 5'

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

to get 3xFLAG Dna2 out cut with BAMH1 SAL1; this vector expresses hDna2 nuclease deficient