pLKO.1p shDna2
(Plasmid
#31951)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 31951 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLKO.1 puro
- Backbone size w/o insert (bp) 7200
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Vector typeMammalian Expression, Lentiviral, RNAi
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameshRNA against hDna2
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Alt nameDna2L
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gRNA/shRNA sequence5’-CATAGCCAGTAGTATTCGATG-3’
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SpeciesH. sapiens (human)
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GenBank IDNM_001080449
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Entrez GeneDNA2 (a.k.a. DNA2L, hDNA2)
- Promoter U6
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Age1 (not destroyed)
- 3′ cloning site EcoR1 (not destroyed)
- 5′ sequencing primer NA (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLKO.1p shDna2 was a gift from Sheila Stewart (Addgene plasmid # 31951 ; http://n2t.net/addgene:31951 ; RRID:Addgene_31951) -
For your References section:
Human Dna2 is a nuclear and mitochondrial DNA maintenance protein. Duxin JP, Dao B, Martinsson P, Rajala N, Guittat L, Campbell JL, Spelbrink JN, Stewart SA. Mol Cell Biol. 2009 Aug;29(15):4274-82. Epub 2009 Jun 1. 10.1128/MCB.01834-08 PubMed 19487465