pBAD/HisD-PATagRFP
(Plasmid #31945)

• Depositing Lab: Vladislav Verkhusha
• Publication: Subach et al J Am Chem Soc. 2010 May 12;132(18):6481-91.

Backbone

• Vector backbone: pBAD/His-B
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 3999
• Modifications to backbone: Several amino acids between His-tag and PATagRFP were removed.
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: PATagRFP
• Alt name: photoactivatable dark-to-red TagRFP red fluorescent protein
• Insert Size (bp): 702
• Mutation: R69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H203R/R207I/V218W/C229S compared to TagRFP (but numbering relative to EGFP)
• Promoter: araB
• Tag / Fusion Protein:
  • His (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: pBAD-fwd
• 3′ sequencing primer: pBAD-rev

Resource Information

• Supplemental Documents:
  • Verkhusha lab feature article

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry