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PITX3-2A-eGFP-PGK-Puro
(Plasmid #31943)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31943 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PCR2.1
  • Backbone manufacturer
    invitrogen
  • Backbone size w/o insert (bp) 8010
  • Vector type
    targeting donor
  • Selectable markers
    Puro

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10/P3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PITX3
  • Species
    H. sapiens (human)
  • Entrez Gene
    PITX3 (a.k.a. ASGD1, ASMD, ASOD, CTPP4, CTRCT11, PTX3)
  • Promoter -
  • Tag / Fusion Protein
    • eGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site - (unknown if destroyed)
  • 3′ cloning site - (unknown if destroyed)
  • 5′ sequencing primer M13
  • 3′ sequencing primer M13
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that the Puromycin selection cassette in this plasmid has a R166H mutation. This mutation has been observed in other plasmids and should not have any affect on the function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PITX3-2A-eGFP-PGK-Puro was a gift from Rudolf Jaenisch (Addgene plasmid # 31943 ; http://n2t.net/addgene:31943 ; RRID:Addgene_31943)

  • For your References section:

    Genetic engineering of human pluripotent cells using TALE nucleases. Hockemeyer D, Wang H, Kiani S, Lai CS, Gao Q, Cassady JP, Cost GJ, Zhang L, Santiago Y, Miller JC, Zeitler B, Cherone JM, Meng X, Hinkley SJ, Rebar EJ, Gregory PD, Urnov FD, Jaenisch R. Nat Biotechnol. 2011 Jul 7. doi: 10.1038/nbt.1927. 10.1038/nbt.1927 PubMed 21738127
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