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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 31942 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePCR2.1
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Backbone manufacturerinvitrogen
- Backbone size w/o insert (bp) 8010
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Vector typetargeting donor
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Selectable markersPuro
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10/P3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePITX3
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SpeciesH. sapiens (human)
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Entrez GenePITX3 (a.k.a. ASGD1, ASMD, ASOD, CTPP4, CTRCT11, PTX3)
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Tag
/ Fusion Protein
- eGFP (C terminal on insert)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PITX3-eGFP-PGK-Puro was a gift from Rudolf Jaenisch (Addgene plasmid # 31942 ; http://n2t.net/addgene:31942 ; RRID:Addgene_31942) -
For your References section:
Genetic engineering of human pluripotent cells using TALE nucleases. Hockemeyer D, Wang H, Kiani S, Lai CS, Gao Q, Cassady JP, Cost GJ, Zhang L, Santiago Y, Miller JC, Zeitler B, Cherone JM, Meng X, Hinkley SJ, Rebar EJ, Gregory PD, Urnov FD, Jaenisch R. Nat Biotechnol. 2011 Jul 7. doi: 10.1038/nbt.1927. 10.1038/nbt.1927 PubMed 21738127