pBAD/HisD-rsTagRFP
(Plasmid #31927)

• Depositing Lab: Vladislav Verkhusha
• Publication: Subach et al Chem Biol. 2010 Jul 30;17(7):745-55.

Backbone

• Vector backbone: pBAD/His-B
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 3999
• Modifications to backbone: Several amino acids between His-tag and rsTagRFP were removed.
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: rsTagRFP
• Alt name: reversibly photoswitchable TagRFP red fluorescent protein
• Insert Size (bp): 702
• Mutation: F84W/I125L/N148A/S165G/F181L/K189N/Y200F compared to TagRFP (but numbering relative to EGFP)
• Promoter: araB
• Tag / Fusion Protein:
  • His (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: pBAD-fwd
• 3′ sequencing primer: pBAD-rev

Resource Information

• Supplemental Documents:
  • Verkhusha lab feature article
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry