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Addgene

prsTagRFP-N1
(Plasmid #31925)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31925 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4013
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    rsTagRFP
  • Alt name
    reversibly photoswitchable TagRFP red fluorescent protein
  • Insert Size (bp)
    702
  • Mutation
    F84W/I125L/N148A/S165G/F181L/K189N/Y200F compared to TagRFP (but numbering relative to EGFP)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    prsTagRFP-N1 was a gift from Vladislav Verkhusha (Addgene plasmid # 31925 ; http://n2t.net/addgene:31925 ; RRID:Addgene_31925)

  • For your References section:

    Red fluorescent protein with reversibly photoswitchable absorbance for photochromic FRET. Subach FV, Zhang L, Gadella TW, Gurskaya NG, Lukyanov KA, Verkhusha VV. Chem Biol. 2010 Jul 30;17(7):745-55. 10.1016/j.chembiol.2010.05.022 PubMed 20659687
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