pBAD/HisB-Slow-FT
(Plasmid #31918)

• Depositing Lab: Vladislav Verkhusha
• Publication: Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epub 2009 Jan 11.

Backbone

• Vector backbone: pBAD/His-B
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4074
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Slow-FT
• Alt name: blue-to-red fluorescent timer
• Insert Size (bp): 711
• Mutation: M18V/E30V/K69R/L84W/A179V compared to mCherry (but numbering relative to EGFP)
• Promoter: araB
• Tag / Fusion Protein:
  • His (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: pBAD-fwd
• 3′ sequencing primer: pBAD-rev

Resource Information

• Supplemental Documents:
  • Verkhusha lab feature article

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry