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Addgene

pBAD/HisB-Medium-FT
(Plasmid #31917)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31917 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD/His-B
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4074
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Medium-FT
  • Alt name
    blue-to-red fluorescent timer
  • Insert Size (bp)
    711
  • Mutation
    M18L/N23D/T43S/K69R/L84W/M152I/Q194L/L205M/Y221C/R227H compared to mCherry (but numbering relative to EGFP)
  • Promoter araB
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer pBAD-fwd
  • 3′ sequencing primer pBAD-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBAD/HisB-Medium-FT was a gift from Vladislav Verkhusha (Addgene plasmid # 31917 ; http://n2t.net/addgene:31917 ; RRID:Addgene_31917)

  • For your References section:

    Monomeric fluorescent timers that change color from blue to red report on cellular trafficking. Subach FV, Subach OM, Gundorov IS, Morozova KS, Piatkevich KD, Cuervo AM, Verkhusha VV. Nat Chem Biol. 2009 Feb;5(2):118-26. Epub 2009 Jan 11. 10.1038/nchembio.138 PubMed 19136976
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