pBAD/HisD-LSSmKate2
(Plasmid #31909)

• Depositing Lab: Vladislav Verkhusha
• Publication: Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;107(12):5369-74. Epub 2010 Mar 8.

Backbone

• Vector backbone: pBAD/His-B
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 3999
• Modifications to backbone: Several amino acids between His-tag and LSSmKate2 were removed.
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: LSSmKate2
• Insert Size (bp): 708
• Mutation: K69Y/P131T/S148G/M167D/T183S/M196V compared to mKate but numbering is relative to EGFP.
• Tag / Fusion Protein:
  • HIS (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pBAD-fwd
• 3′ sequencing primer: pBAD-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry