pDest-mCherry-N1
(Plasmid #31907)

• Purpose: (Empty Backbone)
• Depositing Lab: Robin Shaw
• Publication: Hong et al PLoS Biol. 2010 Feb . 8(2):e1000312.

Backbone

• Vector backbone: pmcherry-N1
• Backbone manufacturer: Clontech
• Backbone size (bp): 4722
• Modifications to backbone: Gateway reading frame cassette c.1 inserted between Afe I and Sma I sites of MCS.
• Vector type: Mammalian Expression
• Promoter: CMV
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Kanamycin, 25 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: High Copy

Resource Information

• Articles Citing this Plasmid:
  • 14 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: the orientation of the MCS within this plasmid places mCherry on the c-terminus of your gene of interest.

Information for Cloning Grade DNA (Catalog # 31907-DNA.cg) ( Back to top)

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

• Amount: 2 µg
• Guaranteed Concentration: 100 ng/µl +/- 5 ng/µl
• Pricing: $105 USD
• Storage: DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here