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Addgene

pMA6-TAF4-3xFLAG
(Plasmid #31901)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31901 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMA6
  • Vector type
    Insect Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TAF4
  • Species
    D. melanogaster (fly)
  • Entrez Gene
    Taf4 (a.k.a. Dmel_CG5444, CG5444, Dmel\CG5444, SR3-3, SY3-2 TAF110, TAF, TAF110, TAF4, TAFII110, TAF[II]110, TAF[[II]], TAF[[II]]110, TFIID, TFIID 110, Taf110, Taf[[II]]110, d110, dTAF4, dTAFII110, dTAF[[II]]110, dmTAF4, l(3)72Dj, p110, taf110)
  • Promoter Actin 5C
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site none (unknown if destroyed)
  • 3′ cloning site none (unknown if destroyed)
  • 5′ sequencing primer AC5
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Previously used in Drosophila, not tested in Sf9 cells.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMA6-TAF4-3xFLAG was a gift from Robert Tjian (Addgene plasmid # 31901 ; http://n2t.net/addgene:31901 ; RRID:Addgene_31901)

  • For your References section:

    Wnt signaling targets ETO coactivation domain of TAF4/TFIID in vivo. Wright KJ, Tjian R. Proc Natl Acad Sci U S A. 2009 Jan 6;106(1):55-60. Epub 2008 Dec 30. 10.1073/pnas.0811914106 PubMed 19116271
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