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pMT-Pygopus
(Plasmid #31900)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31900 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMT-V5/HIS
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3538
  • Vector type
    Insect Expression
  • Selectable markers
    See comments

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pygopus
  • Species
    D. melanogaster (fly)
  • Entrez Gene
    pygo (a.k.a. Dmel_CG11518, BEST:LD21971, CG11518, Dm Pygo, Dmel\CG11518, Pygo, anon-WO02077023.1, dPygo, gam, pNB5.2, pyg, pypo)
  • Promoter P-MT (Metallothionein)
  • Tags / Fusion Proteins
    • V5 (C terminal on backbone)
    • 6xHIS (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site none (unknown if destroyed)
  • 3′ cloning site none (unknown if destroyed)
  • 5′ sequencing primer Metallothionein
  • 3′ sequencing primer EBV reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Blasticidin OR Hygromycin selection

Previously used in Drosophila, not tested in Sf9 cells.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMT-Pygopus was a gift from Robert Tjian (Addgene plasmid # 31900 ; http://n2t.net/addgene:31900 ; RRID:Addgene_31900)

  • For your References section:

    Wnt signaling targets ETO coactivation domain of TAF4/TFIID in vivo. Wright KJ, Tjian R. Proc Natl Acad Sci U S A. 2009 Jan 6;106(1):55-60. Epub 2008 Dec 30. 10.1073/pnas.0811914106 PubMed 19116271
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