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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 31895 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1
- Backbone size w/o insert (bp) 5428
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFrataxin
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SpeciesH. sapiens (human)
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Entrez GeneFXN (a.k.a. CyaY, FA, FARR, FRDA, X25)
- Promoter CMV
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Tag
/ Fusion Protein
- HA (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site none (unknown if destroyed)
- 3′ cloning site none (unknown if destroyed)
- 5′ sequencing primer T7 fwd
- 3′ sequencing primer BGH rev (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1-hFrataxin-HA was a gift from Gino Cortopassi (Addgene plasmid # 31895 ; http://n2t.net/addgene:31895 ; RRID:Addgene_31895) -
For your References section:
Mitochondrial frataxin interacts with ISD11 of the NFS1/ISCU complex and multiple mitochondrial chaperones. Shan Y, Napoli E, Cortopassi G. Hum Mol Genet. 2007 Apr 15;16(8):929-41. Epub 2007 Mar 1. 10.1093/hmg/ddm038 PubMed 17331979