pBAD-PSmOrange
(Plasmid #31886)

• Depositing Lab: Vladislav Verkhusha
• Publication: Subach et al Nat Methods. 2011 Jul 31;8(9):771-7. doi: 10.1038/nmeth.1664.

Backbone

• Vector backbone: pBAD
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4100
• Modifications to backbone: ATGGGGAGCCACCATCACCATCACCATGGCAGATCT instead of ATGGGGGGTTCTCATCATCATCATCATCATGGTATGGCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGAGCTCG
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10/P3
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: PSmOrange
• Insert Size (bp): 711
• Mutation: S21T, Q63L, F100Y, L125M, K166R, P192S (mutations are relative to mOrange but numbering is relative to EGFP)
• GenBank ID: JN_376081

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pBAD-fwd
• 3′ sequencing primer: pBAD-rev

Resource Information

• Supplemental Documents:
  • Verkhusha lab feature article
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry