psiCHECK-2-TGFBR2-m3_3'UTR
(Plasmid #31883)

• Depositing Lab: Robert Blelloch
• Publication: Subramanyam et al Nat Biotechnol. 2011 May;29(5):443-8. Epub 2011 Apr 13.

Backbone

• Vector backbone: psiCHECK-2
• Backbone size w/o insert (bp): 6273
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TGFBR2 mutant 3'UTR
• Species: H. sapiens (human)
• Mutation: Third miR-302b, 372 7-mer seed match mutated from AGCACTT to AGAAATT
• Entrez Gene: TGFBR2 (a.k.a. AAT3, FAA3, LDS1B, LDS2, LDS2B, MFS2, RIIC, TAAD2, TBR-ii, TBRII, TGFR-2, TGFbeta-RII, tbetaR-II)
• Promoter: SV40
• Tag / Fusion Protein:
  • Luciferase (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: psiCHECK2-F

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry