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psiCHECK-2-Sal4-wt_3'UTR
(Plasmid #31862)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31862 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    psiCHECK-2
  • Backbone size w/o insert (bp) 6273
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sal4 wild-type 3'UTR
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1700
  • Promoter SV40
  • Tag / Fusion Protein
    • Luciferase (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer psiCHECK2-F
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    psiCHECK-2-Sal4-wt_3'UTR was a gift from Robert Blelloch (Addgene plasmid # 31862 ; http://n2t.net/addgene:31862 ; RRID:Addgene_31862)

  • For your References section:

    Opposing microRNA families regulate self-renewal in mouse embryonic stem cells. Melton C, Judson RL, Blelloch R. Nature. 2010 Feb 4;463(7281):621-6. Epub 2010 Jan 6. 10.1038/nature08725 PubMed 20054295
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