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pShuttle-CMV-iRFP
(Plasmid #31856)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31856 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pShuttle-CMV
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 7384
  • Vector type
    Mammalian Expression, Adenoviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    iRFP
  • Alt name
    iRFP713
  • Species
    Rhodopseudomonas palustris
  • Insert Size (bp)
    948
  • Mutation
    T2A, S13L, A92T, V104I, V114I, E161K, Y193K, F198Y, D202T, I203V, Y258F, A283V, K288T and N290Y relative to RpBphP2
  • GenBank ID
    JN247409
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For additional protocols on using iRFP in other tissue types please review:
Verkhusha. Deep-tissue photoacoustic tomography of a genetically encoded near-infrared fluorescent probe. Angewandte Chemie Int. Ed. 2012, 51: 1448-1451.
PubMed: https://www.ncbi.nlm.nih.gov/pubmed/22213541/

For more information on in vivo imaging, please see:
https://www.addgene.org/fluorescent_proteins/in_vivo/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pShuttle-CMV-iRFP was a gift from Vladislav Verkhusha (Addgene plasmid # 31856 ; http://n2t.net/addgene:31856 ; RRID:Addgene_31856)

  • For your References section:

    Bright and stable near-infrared fluorescent protein for in vivo imaging. Filonov GS, Piatkevich KD, Ting LM, Zhang J, Kim K, Verkhusha VV. Nat Biotechnol. 2011 Jul 17. doi: 10.1038/nbt.1918. 10.1038/nbt.1918 PubMed 21765402
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