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pBAD/His-B-iRFP
(Plasmid #31855)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31855 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD/His-B
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4100
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    iRFP
  • Alt name
    iRFP713
  • Species
    Rhodopseudomonas palustris
  • Insert Size (bp)
    948
  • Mutation
    S13L, A92T, V104I, V114I, E161K, Y193K, F198Y, D202T, I203V, Y258F, A283V, K288T and N290Y relative to RpBphP2
  • GenBank ID
    JN247409
  • Promoter araBAD

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer pBAD_fwd_primer
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For additional protocols on using iRFP in other tissue types please review:
Verkhusha. Deep-tissue photoacoustic tomography of a genetically encoded near-infrared fluorescent probe. Angewandte Chemie Int. Ed. 2012, 51: 1448-1451.
PubMed: https://www.ncbi.nlm.nih.gov/pubmed/22213541

For more information on in vivo imaging, please see:
https://www.addgene.org/fluorescent_proteins/in_vivo/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBAD/His-B-iRFP was a gift from Vladislav Verkhusha (Addgene plasmid # 31855 ; http://n2t.net/addgene:31855 ; RRID:Addgene_31855)

  • For your References section:

    Bright and stable near-infrared fluorescent protein for in vivo imaging. Filonov GS, Piatkevich KD, Ting LM, Zhang J, Kim K, Verkhusha VV. Nat Biotechnol. 2011 Jul 17. doi: 10.1038/nbt.1918. 10.1038/nbt.1918 PubMed 21765402
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