pSC301b
(Plasmid
#31852)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 31852 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSODIT
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Backbone manufacturerKoen De Smet Imperial College Medical School
- Backbone size w/o insert (bp) 5304
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Hygromycin, 200 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGFP (including ATG start codon)
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Insert Size (bp)723
- Promoter none
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (destroyed during cloning)
- 3′ cloning site HindIII (destroyed during cloning)
- 5′ sequencing primer NA
- 3′ sequencing primer GFP-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid can also be grown in mycobacteria.
This is a control plasmid for pSC301 in which the SOD promoter was excised.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSC301b was a gift from Yossef Av-Gay (Addgene plasmid # 31852 ; http://n2t.net/addgene:31852 ; RRID:Addgene_31852) -
For your References section:
Monitoring promoter activity and protein localization in Mycobacterium spp. using green fluorescent protein. Cowley SC, Av-Gay Y. Gene. 2001 Feb 21;264(2):225-31. 10.1016/S0378-1119(01)00336-5 PubMed 11250077