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Addgene

pSicoR-Ef1a-mCh-Puro Notes

Addgene diagnostic digest

This gel depicts the NEB 1kb extend DNA ladder and a digestion of plasmid 31845 with XhoI and EcoRI-HF.

We expected the following banding patterns:

Single digests - 8141bp. Double digest - 1964bp and 6177bp

Our stock shows the expected banding pattern.