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pIRESpuro2-UBXD1-3x HA
(Plasmid #31836)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31836 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRESpuro2
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5200
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    UBXD1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1300
  • GenBank ID
    NP_079517
  • Entrez Gene
    UBXN6 (a.k.a. UBXD1, UBXDC2)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3x HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

UBXD1 cloned into the pIRESpuro2 vector with 5'EcoRI-BamHI3' and 3x HA tag cloned separately with 5'BamHI-NotI3'.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIRESpuro2-UBXD1-3x HA was a gift from Hemmo Meyer (Addgene plasmid # 31836 ; http://n2t.net/addgene:31836 ; RRID:Addgene_31836)

  • For your References section:

    Endolysosomal sorting of ubiquitylated caveolin-1 is regulated by VCP and UBXD1 and impaired by VCP disease mutations. Ritz D, Vuk M, Kirchner P, Bug M, Schutz S, Hayer A, Bremer S, Lusk C, Baloh RH, Lee H, Glatter T, Gstaiger M, Aebersold R, Weihl CC, Meyer H. Nat Cell Biol. 2011 Aug 7;13(9):1116-23. doi: 10.1038/ncb2301. 10.1038/ncb2301 PubMed 21822278
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