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Purpose(Empty Backbone)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 31830 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepT3TS
- Backbone size (bp) 3216
- Promoter Lac
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pT3TS was a gift from Stephen Ekker (Addgene plasmid # 31830 ; http://n2t.net/addgene:31830 ; RRID:Addgene_31830) -
For your References section:
Vectors and techniques for ectopic gene expression in zebrafish. Hyatt TM, Ekker SC. Methods Cell Biol. 1999;59:117-26. PubMed 9891358