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Addgene

pminiTol2 Notes

Addgene diagnostic digest

This gel depicts the NEB 1kb DNA ladder, the NEB 100bp DNA ladder, and a digestion of plasmid 31829 with EcoRI-HF and MluI-HF.

Our digests confirmed the expected banding patterns:

EcoRI-HF single digest: 3.5 KB

MluI-HF single digest: 3.5 KB

EcoRI-HF / MluI-HF double digest: 3.25 and 0.25 KB.