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Purpose(Empty Backbone)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 31828 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGBT
- Backbone size (bp) 9119
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Vector typeCre/Lox
- Promoter beta Actin
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Tags
/ Fusion Proteins
- mRFP (N terminal on backbone)
- GFP GM2 variant (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The plasmid contains Tol2 transposon mini ITRs.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGBT-RP2-1 was a gift from Stephen Ekker (Addgene plasmid # 31828 ; http://n2t.net/addgene:31828 ; RRID:Addgene_31828) -
For your References section:
In vivo protein trapping produces a functional expression codex of the vertebrate proteome. Clark KJ, Balciunas D, Pogoda HM, Ding Y, Westcot SE, Bedell VM, Greenwood TM, Urban MD, Skuster KJ, Petzold AM, Ni J, Nielsen AL, Patowary A, Scaria V, Sivasubbu S, Xu X, Hammerschmidt M, Ekker SC. Nat Methods. 2011 Jun;8(6):506-15. Epub 2011 May 8. 10.1038/nmeth.1606 PubMed 21552255