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Addgene

pGBT-RP2-1
(Plasmid #31828)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31828 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGBT
  • Backbone size (bp) 9119
  • Vector type
    Cre/Lox
  • Promoter beta Actin
  • Tags / Fusion Proteins
    • mRFP (N terminal on backbone)
    • GFP GM2 variant (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The plasmid contains Tol2 transposon mini ITRs.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGBT-RP2-1 was a gift from Stephen Ekker (Addgene plasmid # 31828 ; http://n2t.net/addgene:31828 ; RRID:Addgene_31828)
  • For your References section:

    In vivo protein trapping produces a functional expression codex of the vertebrate proteome. Clark KJ, Balciunas D, Pogoda HM, Ding Y, Westcot SE, Bedell VM, Greenwood TM, Urban MD, Skuster KJ, Petzold AM, Ni J, Nielsen AL, Patowary A, Scaria V, Sivasubbu S, Xu X, Hammerschmidt M, Ekker SC. Nat Methods. 2011 Jun;8(6):506-15. Epub 2011 May 8. 10.1038/nmeth.1606 PubMed 21552255