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Addgene

pGBT-R16
(Plasmid #31827)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31827 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDB685
  • Backbone size w/o insert (bp) 5622
  • Vector type
    Cre/Lox ; gene trap

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mini Tol2 ITR
  • Alt name
    Tol2 transposon
  • Species
    Artificial sequence
  • Mutation
    mRFP lacks start codon
  • Tag / Fusion Protein
    • mRFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NA (unknown if destroyed)
  • 3′ cloning site NA (unknown if destroyed)
  • 5′ sequencing primer M13pUC-rev
  • 3′ sequencing primer M13pUC-fwd
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGBT-R16 was a gift from Stephen Ekker (Addgene plasmid # 31827 ; http://n2t.net/addgene:31827 ; RRID:Addgene_31827)

  • For your References section:

    In vivo protein trapping produces a functional expression codex of the vertebrate proteome. Clark KJ, Balciunas D, Pogoda HM, Ding Y, Westcot SE, Bedell VM, Greenwood TM, Urban MD, Skuster KJ, Petzold AM, Ni J, Nielsen AL, Patowary A, Scaria V, Sivasubbu S, Xu X, Hammerschmidt M, Ekker SC. Nat Methods. 2011 Jun;8(6):506-15. Epub 2011 May 8. 10.1038/nmeth.1606 PubMed 21552255
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