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Addgene

pGBT-R15
(Plasmid #31826)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31826 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGBT
  • Vector type
    Cre/Lox ; gene trap

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Tol2
  • Species
    D. rerio (zebrafish)
  • Entrez Gene
    t2gtlp4 (a.k.a. SAGp4)
  • Tag / Fusion Protein
    • mRFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NA (unknown if destroyed)
  • 3′ cloning site NA (unknown if destroyed)
  • 5′ sequencing primer M13pUC-rev
  • 3′ sequencing primer M13pUC-fwd
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGBT-R15 was a gift from Stephen Ekker (Addgene plasmid # 31826 ; http://n2t.net/addgene:31826 ; RRID:Addgene_31826)

  • For your References section:

    Nicotine response genetics in the zebrafish. Petzold AM, Balciunas D, Sivasubbu S, Clark KJ, Bedell VM, Westcot SE, Myers SR, Moulder GL, Thomas MJ, Ekker SC. Proc Natl Acad Sci U S A. 2009 Nov 3;106(44):18662-7. Epub 2009 Oct 26. 10.1073/pnas.0908247106 PubMed 19858493
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