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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 31821 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5400
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameYFP-Protein Kinase D2
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Alt nameYFP-PKD2
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Alt namePKD2
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SpeciesH. sapiens (human)
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Insert Size (bp)2655
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GenBank IDAF309082.1
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Entrez GenePRKD2 (a.k.a. HSPC187, PKD2, nPKC-D2)
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Tag
/ Fusion Protein
- YFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer Sp6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-YFP-PKD2 was a gift from Alexandra Newton (Addgene plasmid # 31821 ; http://n2t.net/addgene:31821 ; RRID:Addgene_31821) -
For your References section:
The protein scaffold NHERF-1 controls the amplitude and duration of localized protein kinase D activity. Kunkel MT, Garcia EL, Kajimoto T, Hall RA, Newton AC. J Biol Chem. 2009 Sep 4. 284(36):24653-61. 10.1074/jbc.M109.024547 PubMed 19581308
Map uploaded by the depositor.
