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Addgene

pcDNA3-YFP-PKD2
(Plasmid #31821)

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31821 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    YFP-Protein Kinase D2
  • Alt name
    YFP-PKD2
  • Alt name
    PKD2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2655
  • GenBank ID
    AF309082.1
  • Entrez Gene
    PRKD2 (a.k.a. HSPC187, PKD2, nPKC-D2)
  • Tag / Fusion Protein
    • YFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer Sp6
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-YFP-PKD2 was a gift from Alexandra Newton (Addgene plasmid # 31821 ; http://n2t.net/addgene:31821 ; RRID:Addgene_31821)
  • For your References section:

    The protein scaffold NHERF-1 controls the amplitude and duration of localized protein kinase D activity. Kunkel MT, Garcia EL, Kajimoto T, Hall RA, Newton AC. J Biol Chem. 2009 Sep 4. 284(36):24653-61. 10.1074/jbc.M109.024547 PubMed 19581308