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Addgene

pSC300
(Plasmid #31820)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 31820 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSODIT
  • Backbone manufacturer
    Koen De Smet Imperial College Medical School
  • Backbone size w/o insert (bp) 5497
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Hygromycin, 200 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP (lacking ATG start codon)
  • Insert Size (bp)
    723
  • Promoter SOD

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (destroyed during cloning)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer NA
  • 3′ sequencing primer GFP-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid can also be grown in mycobacteria.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSC300 was a gift from Yossef Av-Gay (Addgene plasmid # 31820 ; http://n2t.net/addgene:31820 ; RRID:Addgene_31820)

  • For your References section:

    Monitoring promoter activity and protein localization in Mycobacterium spp. using green fluorescent protein. Cowley SC, Av-Gay Y. Gene. 2001 Feb 21;264(2):225-31. 10.1016/S0378-1119(01)00336-5 PubMed 11250077
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