RSRF-Luc-2mut
(Plasmid #31819)

• Depositing Lab: Astar Winoto
• Publication: Woronicz et al Mol Cell Biol. 1995 Nov;15(11):6364-76.

Backbone

• Vector backbone: pGL2-basic
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 5600
• Modifications to backbone: The minimal c-fos promoter fragment was cloned into the SmaI and BglII sites of pGL2 basic.
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Mutant MEF2-dependent promoter (-307 to -242 of Nur77/NR4A1)
• Species: M. musculus (mouse)
• Mutation: two CTATATTTAG RSRF sites mutated to CGATATTTCG
• Promoter: mutated MEF2-dependent (-307 to -242 of Nur77/NR4A1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (unknown if destroyed)
• 3′ cloning site: SalI (unknown if destroyed)
• 5′ sequencing primer: EBV-reverse
• 3′ sequencing primer: LucNrev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The minimal c-fos promoter fragment was cloned into the SmaI and BglII sites of pGL2 basic. The mutated RSRF sequence (two CTATATTTAG RSRF sites mutated to CGATATTTCG) of the -307 to -242 Nur77/NR4A1 promoter region was cloned in to the SalI site of this SmaI/BglII fragment.