RSRF-Luc-2wt
(Plasmid #31818)

• Depositing Lab: Astar Winoto
• Publication: Woronicz et al Mol Cell Biol. 1995 Nov;15(11):6364-76.

Backbone

• Vector backbone: pGL2-basic
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 5600
• Modifications to backbone: The minimal c-fos promoter fragment was cloned into the SmaI and BglII sites of pGL2 basic.
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MEF2-dependent promoter (-307 to -242 of Nur77/NR4A1)
• Species: M. musculus (mouse)
• Promoter: MEF2-dependent (-307 to -242 of Nur77/NR4A1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (unknown if destroyed)
• 3′ cloning site: SalI (unknown if destroyed)
• 5′ sequencing primer: EBV-reverse
• 3′ sequencing primer: LucNrev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The minimal c-fos promoter fragment was cloned into the SmaI and BglII sites of pGL2 basic. The wild-type RSRF sequence of the -307 to -242 Nur77/NR4A1 promoter region was cloned in to the SalI site of this SmaI/BglII fragment.