pCI-His-hUbi
(Plasmid #31815)

• Depositing Lab: Astar Winoto
• Publication: Young et al J Biol Chem. 2011 Feb 25;286(8):6521-31. Epub 2010 Dec 23.

Backbone

• Vector backbone: pCI
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ubiquitin
• Alt name: UBB
• Species: H. sapiens (human)
• Entrez Gene: UBB (a.k.a. HEL-S-50)
• Promoter: CMV
• Tag / Fusion Protein:
  • HIS (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: EBV reverse

Resource Information

• Articles Citing this Plasmid:
  • 23 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Ubiquitin was PCR amplified from pcDNA3-HAUbiquitin (Addgene #18712) for cloning into this vector. As noted on the plasmid page for #18712: "The HA-Ubiquitin insert is from the UBB gene. The reason for the small size is because it contains a single ubiquitin coding sequence (full length UBB has three repeating ubiquitin coding sequences)."