pTRE-TIGHT-Cx43-eYFP
(Plasmid #31807)

• Depositing Lab: Robin Shaw
• Publication: Smyth et al J Clin Invest. 2010 Jan 4. 120(1):266-79.

Backbone

• Vector backbone: pTRE-TIGHT
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 2600
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Cx43
• Species: H. sapiens (human)
• Entrez Gene: GJA1 (a.k.a. AVSD3, CMDR, CX43, EKVP, EKVP3, GJAL, HLHS1, HSS, ODDD, PPKCA)
• Promoter: P-TIGHT
• Tag / Fusion Protein:
  • eYFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: LNCX_primer
• 3′ sequencing primer: EXFP-R

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Human Cx43 was initially cloned into pEYFP-N1 using the NheI and HindIII cut sites of this vector. Cx43-EYFP was then excised from pEYFP-N1 using NheI and NotI to insert the fusion protein encoding genes into pTRE-Tight, so importantly, some of the MCS from pEYFP-N1 remains in the final construct.